polr2a, ppib and ubc 3-plex positive control probes Search Results


rnascope multiplex fluorescent v2 assay  (Advanced Cell Diagnostics Inc)
99
Advanced Cell Diagnostics Inc rnascope multiplex fluorescent v2 assay
(A, E) mRNA transcript expression visualized by <t>RNAscope</t> hybridization with probes for (A) Scnn1a (green, upper left), Tmprss2 (red channel, bottom left), and Scnn1a-Tmprss2 merged picture with DAPI counterstain (middle) in the cortex, medulla, and papilla (right panels). (B, C, D) Quantification of total cells double-positive (DP) for both Scnn1a and Tmprss2 , single-positive (SP), or double-negative (DN) in (B) the cortex and (C) medulla. (D, H) Relative (D) Tmprss2 and (H) Furin intensity in DP and SP of the cortex and medulla from male and female mice. (E) RNAscope analysis using probes for Scnn1a (green, upper left), Furin (red, bottom left), and Scnn1a-Furin merged picture with DAPI counterstain (middle) with insets for cortex, medulla, and papilla (right panels). (F, G, H) Quantification of total cells double-positive (DP) for both Scnn1a and Furin , single-positive (SP), or double-negative (DN) in (F) the cortex, (G) medulla, and (H) cortex and medulla from male and female mice. 12 kidneys (six cut transversally and six cut longitudinally) from three male and three female C57BL/6J mice were analysed. Scale bar: 200 μm. Experiments are represented as the mean ± SEM. Data were analysed by an unpaired two-tailed Welch t test, and P -values < 0.05 were considered statistically significant; *** P < 0.001. Source data are available for this figure.
Rnascope Multiplex Fluorescent V2 Assay, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polr2a%2C+ppib+and+ubc+3-plex+positive+control+probes/RNAscope+Multiplex+Fluorescent+Reagent+Kit+v2/pmc10765116-231-1-23
Average 99 stars, based on 1 article reviews
rnascope multiplex fluorescent v2 assay - by Bioz Stars, 2026-10
99/100 stars
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absorbent paper  (Danaher Inc)
91
Danaher Inc absorbent paper
(A, E) mRNA transcript expression visualized by <t>RNAscope</t> hybridization with probes for (A) Scnn1a (green, upper left), Tmprss2 (red channel, bottom left), and Scnn1a-Tmprss2 merged picture with DAPI counterstain (middle) in the cortex, medulla, and papilla (right panels). (B, C, D) Quantification of total cells double-positive (DP) for both Scnn1a and Tmprss2 , single-positive (SP), or double-negative (DN) in (B) the cortex and (C) medulla. (D, H) Relative (D) Tmprss2 and (H) Furin intensity in DP and SP of the cortex and medulla from male and female mice. (E) RNAscope analysis using probes for Scnn1a (green, upper left), Furin (red, bottom left), and Scnn1a-Furin merged picture with DAPI counterstain (middle) with insets for cortex, medulla, and papilla (right panels). (F, G, H) Quantification of total cells double-positive (DP) for both Scnn1a and Furin , single-positive (SP), or double-negative (DN) in (F) the cortex, (G) medulla, and (H) cortex and medulla from male and female mice. 12 kidneys (six cut transversally and six cut longitudinally) from three male and three female C57BL/6J mice were analysed. Scale bar: 200 μm. Experiments are represented as the mean ± SEM. Data were analysed by an unpaired two-tailed Welch t test, and P -values < 0.05 were considered statistically significant; *** P < 0.001. Source data are available for this figure.
Absorbent Paper, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polr2a%2C+ppib+and+ubc+3-plex+positive+control+probes/GB003+110x140MM+100%2FPK/pmc07476812-105-116-119
Average 91 stars, based on 1 article reviews
absorbent paper - by Bioz Stars, 2026-10
91/100 stars
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1x pbs rnase free hydrophobic barrier pen  (Vector Laboratories)
96
Vector Laboratories 1x pbs rnase free hydrophobic barrier pen
(A, E) mRNA transcript expression visualized by <t>RNAscope</t> hybridization with probes for (A) Scnn1a (green, upper left), Tmprss2 (red channel, bottom left), and Scnn1a-Tmprss2 merged picture with DAPI counterstain (middle) in the cortex, medulla, and papilla (right panels). (B, C, D) Quantification of total cells double-positive (DP) for both Scnn1a and Tmprss2 , single-positive (SP), or double-negative (DN) in (B) the cortex and (C) medulla. (D, H) Relative (D) Tmprss2 and (H) Furin intensity in DP and SP of the cortex and medulla from male and female mice. (E) RNAscope analysis using probes for Scnn1a (green, upper left), Furin (red, bottom left), and Scnn1a-Furin merged picture with DAPI counterstain (middle) with insets for cortex, medulla, and papilla (right panels). (F, G, H) Quantification of total cells double-positive (DP) for both Scnn1a and Furin , single-positive (SP), or double-negative (DN) in (F) the cortex, (G) medulla, and (H) cortex and medulla from male and female mice. 12 kidneys (six cut transversally and six cut longitudinally) from three male and three female C57BL/6J mice were analysed. Scale bar: 200 μm. Experiments are represented as the mean ± SEM. Data were analysed by an unpaired two-tailed Welch t test, and P -values < 0.05 were considered statistically significant; *** P < 0.001. Source data are available for this figure.
1x Pbs Rnase Free Hydrophobic Barrier Pen, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polr2a%2C+ppib+and+ubc+3-plex+positive+control+probes/ImmEdge+Hydrophobic+Barrier+PAP+Pen/pmc07476812-105-100-111
Average 96 stars, based on 1 article reviews
1x pbs rnase free hydrophobic barrier pen - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


(A, E) mRNA transcript expression visualized by RNAscope hybridization with probes for (A) Scnn1a (green, upper left), Tmprss2 (red channel, bottom left), and Scnn1a-Tmprss2 merged picture with DAPI counterstain (middle) in the cortex, medulla, and papilla (right panels). (B, C, D) Quantification of total cells double-positive (DP) for both Scnn1a and Tmprss2 , single-positive (SP), or double-negative (DN) in (B) the cortex and (C) medulla. (D, H) Relative (D) Tmprss2 and (H) Furin intensity in DP and SP of the cortex and medulla from male and female mice. (E) RNAscope analysis using probes for Scnn1a (green, upper left), Furin (red, bottom left), and Scnn1a-Furin merged picture with DAPI counterstain (middle) with insets for cortex, medulla, and papilla (right panels). (F, G, H) Quantification of total cells double-positive (DP) for both Scnn1a and Furin , single-positive (SP), or double-negative (DN) in (F) the cortex, (G) medulla, and (H) cortex and medulla from male and female mice. 12 kidneys (six cut transversally and six cut longitudinally) from three male and three female C57BL/6J mice were analysed. Scale bar: 200 μm. Experiments are represented as the mean ± SEM. Data were analysed by an unpaired two-tailed Welch t test, and P -values < 0.05 were considered statistically significant; *** P < 0.001. Source data are available for this figure.

Journal: Life Science Alliance

Article Title: Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport

doi: 10.26508/lsa.202302304

Figure Lengend Snippet: (A, E) mRNA transcript expression visualized by RNAscope hybridization with probes for (A) Scnn1a (green, upper left), Tmprss2 (red channel, bottom left), and Scnn1a-Tmprss2 merged picture with DAPI counterstain (middle) in the cortex, medulla, and papilla (right panels). (B, C, D) Quantification of total cells double-positive (DP) for both Scnn1a and Tmprss2 , single-positive (SP), or double-negative (DN) in (B) the cortex and (C) medulla. (D, H) Relative (D) Tmprss2 and (H) Furin intensity in DP and SP of the cortex and medulla from male and female mice. (E) RNAscope analysis using probes for Scnn1a (green, upper left), Furin (red, bottom left), and Scnn1a-Furin merged picture with DAPI counterstain (middle) with insets for cortex, medulla, and papilla (right panels). (F, G, H) Quantification of total cells double-positive (DP) for both Scnn1a and Furin , single-positive (SP), or double-negative (DN) in (F) the cortex, (G) medulla, and (H) cortex and medulla from male and female mice. 12 kidneys (six cut transversally and six cut longitudinally) from three male and three female C57BL/6J mice were analysed. Scale bar: 200 μm. Experiments are represented as the mean ± SEM. Data were analysed by an unpaired two-tailed Welch t test, and P -values < 0.05 were considered statistically significant; *** P < 0.001. Source data are available for this figure.

Article Snippet: The RNAscope Multiplex Fluorescent V2 assay was performed according to the manufacturer’s protocol on 4-μm paraffin sections and hybridized with probes purchased from ACD Bio-Techne: Mm- Scnn1a (#441391-C3); Mm- Tmprss2 (#496721); Mm- Furin (#864041); Mm-3-plex positive control Polr2a-C1 , Ppib-C2 , and Ubc-C3 (#320881); and 3-plex negative control bacterial DapB (#320871); and revealed with TSA Opal570 (#FP1488001KT) or TSA Opal650 (#FP1496001KT).

Techniques: Expressing, RNAscope, Hybridization, Two Tailed Test

(A, B) RNAscope hybridization control probes targeting positive mouse genes Polr2a (green, upper left) and Ppib (red, lower left) (A); negative control probes targeting the bacterial DapB gene in both channels (red and green, left) (B); and DAPI-counterstained merged images (middle). (A, B) Whole kidney images and insets of higher magnification in the renal cortex, medulla, and papilla are shown (A, B) (upper right, middle, and bottom right panels). Scale bar: 200 μM. Source data are available for this figure.

Journal: Life Science Alliance

Article Title: Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport

doi: 10.26508/lsa.202302304

Figure Lengend Snippet: (A, B) RNAscope hybridization control probes targeting positive mouse genes Polr2a (green, upper left) and Ppib (red, lower left) (A); negative control probes targeting the bacterial DapB gene in both channels (red and green, left) (B); and DAPI-counterstained merged images (middle). (A, B) Whole kidney images and insets of higher magnification in the renal cortex, medulla, and papilla are shown (A, B) (upper right, middle, and bottom right panels). Scale bar: 200 μM. Source data are available for this figure.

Article Snippet: The RNAscope Multiplex Fluorescent V2 assay was performed according to the manufacturer’s protocol on 4-μm paraffin sections and hybridized with probes purchased from ACD Bio-Techne: Mm- Scnn1a (#441391-C3); Mm- Tmprss2 (#496721); Mm- Furin (#864041); Mm-3-plex positive control Polr2a-C1 , Ppib-C2 , and Ubc-C3 (#320881); and 3-plex negative control bacterial DapB (#320871); and revealed with TSA Opal570 (#FP1488001KT) or TSA Opal650 (#FP1496001KT).

Techniques: RNAscope, Hybridization, Control, Negative Control